Disney has accused Florida Governor Ron DeSantis of organising a campaign of "government retaliation" in a lawsuit.
The new legal action sharply escalates the battle between the entertainment giant and the Republican politician.
The two sides have been fighting since Disney criticised a state law banning discussion of sexual orientation or gender identity in primary schools.
The lawsuit came after state officials voided a development deal involving the firm's Florida theme park.
Disney said DeSantis' steps to assert control over its operations threatened its business and violated its constitutional rights.
Recently, the Florida House Republicans passed a litany of anti-LGBTQ legislation. These bills could prohibit gender-affirming care for children, punish businesses letting minors watch drag shows, and treat transgender people peeing in the wrong bathroom as criminals.
President Biden denounced Florida legislation restricting LGBTQ rights.
It is time to change Florida!!!